作者: David M. Parichy , Michael R. Elizondo , Margaret G. Mills , Tiffany N. Gordon , Raymond E. Engeszer
DOI: 10.1002/DVDY.22113
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摘要: The zebrafish is a premier model organism yet lacks system for assigning postembryonic fish to developmental stages. To provide such staging series, we describe changes in several traits that are visible under brightfield illumination or through vital staining and epiflourescent illumination. These include the swim bladder, median pelvic fins, pigment pattern, scale formation, larval fin fold, skeleton. We further identify milestones placing into discrete relate these size age show better indicator of progress than age. also examine how relationships between vary with temperature density, document effects histological processing on size. facilitate staging, images reference individuals have attained specific defined sizes. Finally, guidelines reporting stages information both continuous growth development.