Recombinant Parainfluenza Virus 5 Expressing Hemagglutinin of Influenza A Virus H5N1 Protected Mice against Lethal Highly Pathogenic Avian Influenza Virus H5N1 Challenge
作者： Z. Li , A. J. Mooney , J. D. Gabbard , X. Gao , P. Xu
DOI: 10.1128/JVI.02321-12
关键词: Virology 、 Influenza A virus subtype H5N1 、 Biology 、 Influenza A virus 、 Hemagglutinin (influenza) 、 Paramyxoviridae 、 Virus 、 Population 、 H5N1 vaccine 、 Vaccination
摘要: A safe and effective vaccine is the best way to prevent large-scale highly pathogenic avian influenza virus (HPAI) H5N1 outbreaks in human population. The current FDA-approved has serious limitations. more efficacious urgently needed. Parainfluenza 5 (PIV5), a paramyxovirus, not known cause any illness humans. PIV5 an attractive vector. In our studies, single dose of live recombinant expressing hemagglutinin (HA) gene (rPIV5-H5) from subtype provided sterilizing immunity against lethal doses HPAI infection mice. Furthermore, we have examined effect insertion HA at different locations within genome on efficacy PIV5-based vaccine. Interestingly, between leader sequence, de facto promoter PIV5, first viral gene, nucleoprotein (NP), did lead viable virus. Insertion NP next V/phosphorprotein (V/P), led that was defective growth. We found junction small hydrophobic (SH) hemagglutinin-neuraminidase (HN) gave challenge: as low 1,000 PFU sufficient protect challenge work suggests great potential
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