FGF-2-Induced Human Amniotic Mesenchymal Stem Cells Seeded on a Human Acellular Amniotic Membrane Scaffold Accelerated Tendon-to-Bone Healing in a Rabbit Extra-Articular Model.

摘要: Background. FGF-2 (basic fibroblast growth factor) has a positive effect on the proliferation and differentiation of many kinds MSCs. Therefore, it represents an ideal molecule to facilitate tendon-to-bone healing. Nonetheless, no studies have investigated application FGF-2-induced human amniotic mesenchymal stem cells (hAMSCs) accelerate healing in vivo. Objective. The purpose this study was explore chondrogenic hAMSCs vitro combined with acellular membrane (HAAM) scaffold Methods. In vitro, were transfected lentivirus carrying gene, potential for induced by gene assessed using immunofluorescence toluidine blue (TB) staining. HAAM prepared, hematoxylin eosin (HE) staining scanning electron microscopy (SEM) used observe microstructure scaffold. without seeded at density cells/well. Immunofluorescence vimentin phalloidin confirm cell adherence vivo, rabbit extra-articular model created right hind limb 40 New Zealand White rabbits. Grafts mimicking interface (TBI) injury subjected treatment loaded hAMSCs, only, scaffold, special treatment. Macroscopic observation, imageological analysis, histological assessment, biomechanical analysis conducted evaluate after 3 months. Results. cartilage-specific marker overexpression group. displayed netted structure mass extracellular matrix structure. or survived grew well. group treated had narrowest bone tunnel three months as compared other groups. addition, macroscopic scores higher than groups, along best mechanical strength. Conclusion. could model.