A rapid, high-throughput screening method for carriage of methicillin-resistant Staphylococcus aureus.
作者： Hege S. Tunsjø , Benoit Follin-Arbelet , Nina M. Clausen , Yngve Ness , Truls M. Leegaard
DOI: 10.1111/APM.12049
关键词: Lysis buffer 、 Mrsa screening 、 Carriage 、 Staphylococcus aureus 、 Microbiology 、 Medicine 、 Methicillin-resistant Staphylococcus aureus 、 High-throughput screening
摘要: Rapid screening of methicillin-resistant Staphylococcus aureus (MRSA) colonization prior to hospital admittance is important reduce nosocomial infections and health care costs. Molecular detection mecA S. specific target genes has become widely established for this purpose. However, there are still limitations in potential high-throughput the methods described. We have compared time aspects workload four different DNA preparation platforms, resulting an automated simple MRSA method which combines two liquid handling systems a lysis buffer. further transferred our in-house dual real-time PCR fast-PCR protocol, reducing labour spent on these samples minimum.
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