Human uterine mast cells. Isolation, purification, characterization, ultrastructure, and pharmacology.

摘要: As part of an ongoing investigation human mast cell heterogeneity, we have isolated, partially purified, and characterized the uterine compared it with cells isolated from other organs. The average histamine content myometrium leiomyofibroma obtained hysterectomies was 2.1 +/- 0.3 (mean SEM) microgram/g tissue (n = 10), two tissues did not differ significantly. A mild collagenase, hyaluronidase, DNase digestion used to disperse cells, yield 9.5% (range, 0 21%). histamine/uterine 0.2 pg 3), 61 7% (n= 3) survived overnight culture. Early purification efforts Percoll gradients yielded up 80% pure 27 10% 5). Uterine released in response secretogogues anti-IgE A23187 but respond substance P or basophil FMLP, C5a, 12-O-tetradecanoylphorbol-13-acetate. After 1 microgram/ml stimulation, appeared make significant quantities PGD2 (89 26 ng/10(6) n 6) (p less than 0.05), as assayed by RIA. Simultaneously, leukotriene C4 release 45 15 Combined gas-chromatography mass spectroscopy analysis anti-IgE-stimulated supernatants confirmed production PGD2. In pharmacologic studies, isobutyl-methylxanthine isoproterenol blocked anti-IgE-induced release. is similar lung terms arachidonic acid metabolites. Ultrastructurally, contains scroll granules, crystal combined homogeneously dense large lipid bodies, many focal lucencies within them. Particle most frequently present gut mucosal origin, were absent cells. Although certain features are analogous ultrastructure skin combination structures distinctive for