Thermal ecophysiology of gametophytes cultured from invasive Undaria pinnatifida (Harvey) Suringar in coastal California harbors.

摘要: article i nfo Heat shock Hsp70 Kelp Photosynthesis Seaweed Temperature Given the ecological and economic impact of invasive species, knowledge traits that allow kelp Undaria pinnatifida (Harvey) Suringar to expand its range is important for developing prevention eradication efforts. Physiological mechanisms thermotolerance may be as they can influence both local regional patterns distribution abundance. An established measure heat response where proteins (Hsps) are produced in high temperature protect cellular from misfolding degradation. It hypothesized Hsp production varies with developmental stage many U. pinnatifida, gametophytes have been shown a broader growth survival than sporophytes. Thus, we examined up-regulation hsp70 gene compared it values previously measured Fertile sporophytes were collected harbors Monterey, Santa Barbara, Los Angeles, San Diego, California, cultured them laboratory. After being grown controlled light conditions, gametophyte tissue was exposed gradient quantitative real-time PCR used determine relative amounts transcript. Expression extremely low gametophytes. investigation thermal ecophysiology using oxygen evolution measures pulse-amplitude-modulated (PAM) fluorometry at subset temperatures revealed decrease dark-adapted yield increasing 17 31 °C. Photosynthetic rates declined but remained positive all incubation temperatures, while respiration increased up 26 °C then declined. metabolically active well above normal environmental indicating broadly thermotolerant. This tolerance, however, likely conferred by other up- regulation no significant increases expression related observed this study.