A synthetic biology standard for Chinese Hamster Ovary cell genome monitoring and contaminant detection by polymerase chain reaction
作者： Alexander Templar , Douglas Marsh , Darren N. Nesbeth
DOI: 10.1186/S40064-016-3074-8
关键词: Genome 、 Molecular biology 、 Synthetic biology 、 Chinese hamster ovary cell 、 Polymerase chain reaction 、 Monoclonal antibody 、 Industry standard 、 Biology
摘要: Background Chinese Hamster Ovary (CHO) cells are the current industry standard for production of therapeutic monoclonal antibodies at commercial scales. Production optimisation in CHO hinges on analytical technologies such as use polymerase chain reaction (PCR) to quantify genetic factors within genome and detect presence contaminant organisms. PCR-based assays, whilst sensitive accurate, limited by (i) requiring lengthy sample preparation (ii) a lack standardisation.
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John M. S. Bartlett, David Stirling, A short history of the polymerase chain reaction. Methods of Molecular Biology. ,vol. 226, pp. 3- 6 ,(2003) , 10.1385/1-59259-384-4:3
Priscila Falagan-Lotsch, Talíria Silva Lopes, Nívea Ferreira, Nathália Balthazar, Antônio M. Monteiro, Radovan Borojevic, José Mauro Granjeiro, Performance of PCR-based and Bioluminescent assays for mycoplasma detection. Journal of Microbiological Methods. ,vol. 118, pp. 31- 36 ,(2015) , 10.1016/J.MIMET.2015.08.010
Carolyn Kay Lincoln, Michael G. Gabridge, Chapter 4 Cell Culture Contamination: Sources, Consequences, Prevention, and Elimination Animal Cell Culture Methods. ,vol. 57, pp. 49- 65 ,(1998) , 10.1016/S0091-679X(08)61571-X
Franz Clementschitsch, Karl Bayer, Improvement of bioprocess monitoring: development of novel concepts Microbial Cell Factories. ,vol. 5, pp. 19- 19 ,(2006) , 10.1186/1475-2859-5-19
E COOPER, Immunology at a glance (3rd edn) by J. H. L. Playfair, Blackwell Scientific Publications, 1984. E5.00 (35 pages) ISBN 0 632 01322 2 Trends in Biotechnology. ,vol. 3, pp. 214- ,(1985) , 10.1016/0167-7799(85)90051-4
Robert G. Rutledge, A Java Program for LRE-Based Real-Time qPCR that Enables Large-Scale Absolute Quantification PLoS ONE. ,vol. 6, pp. e17636- ,(2011) , 10.1371/JOURNAL.PONE.0017636
Jason R Kelly, Adam J Rubin, Joseph H Davis, Caroline M Ajo-Franklin, John Cumbers, Michael J Czar, Kim de Mora, Aaron L Glieberman, Dileep D Monie, Drew Endy, Measuring the activity of BioBrick promoters using an in vivo reference standard Journal of Biological Engineering. ,vol. 3, pp. 4- 4 ,(2009) , 10.1186/1754-1611-3-4
Yegor Voronin, Sarah Holte, Julie Overbaugh, Michael Emerman, Genetic Drift of HIV Populations in Culture PLOS Genetics. ,vol. 5, ,(2009) , 10.1371/JOURNAL.PGEN.1000431
J. Martin Bland, Douglas G. Altman, Statistical methods for assessing agreement between two methods of clinical measurement International Journal of Nursing Studies. ,vol. 47, pp. 931- 936 ,(2010) , 10.1016/J.IJNURSTU.2009.10.001
C. Kaiser, T. Pototzki, A. Ellert, R. Luttmann, Applications of PAT‐Process Analytical Technology in Recombinant Protein Processes with Escherichia coli Engineering in Life Sciences. ,vol. 8, pp. 132- 138 ,(2008) , 10.1002/ELSC.200720232