Performance of PCR-based and Bioluminescent assays for mycoplasma detection.

摘要: Contaminated eukaryotic cell cultures are frequently responsible for unreliable results. Regulatory entities request that must be mycoplasma-free. Mycoplasma contamination remains a significant problem and may have an impact on biological analysis since they affect many parameters. The gold standard microbiological assay mycoplasma detection involves laborious time-consuming protocols. PCR-based Bioluminescent assays been considered routine culture screening in research laboratories fast, easy sensitive. Thus, the aim of this work is to compare performance two popular commercial assays, by assessing level from Rio de Janeiro Cell Bank (RJCB) also customers' laboratories. results obtained both performed were confirmed scanning electron microscopy. In addition, we evaluated limit PCR kit under our laboratory conditions storage effects frozen supernatants. was not significantly different showed very good agreement. slightly more dependable than due lack inconclusive produced first technique, especially considering ability detect However, lines should precultured four days or without antibiotics obtain safe On other hand, false negative result using biochemical approach. implementation fast reliable testing methods important technical regulatory issue candidates. validation studies needed.