Cloning and Expression of the Lactococcus lactis purDEK Genes, Required for Growth in Milk

摘要: An operon containing the genes purD and purE part of purK gene was cloned from facultative anaerobic gram-positive bacterium Lactococcus lactis by complementation mutation in Escherichia coli SO609. The encode enzymes de novo pathway purine nucleotides. expression regulated approximately 35-fold at transcription level availability purines growth medium. Deletion analysis nucleotide region upstream indicated that a 145 bp is enough to give reporter lacLM genes, which β-galactosidase. 79 start point reduced promoter activity 33-fold when incubated purine-free medium values below detection limit purine-containing No secondary points were mapped or close this region, indicating putative activator site not deleted partly destroyed.