Three-Dimensional Paper-Based Model for Cardiac Ischemia

摘要: In vitro models of ischemia have not historically recapitulated the cellular interactions and gradients molecules that occur in a 3D tissue. This work demonstrates paper-based culture system mimics some among populations cells heart during ischemia. Multiple layers paper containing cells, suspended hydrogels, are stacked to form layered model Mass transport oxygen glucose into this can be modulated induce an ischemic environment bottom stack. stress induces cardiomyocytes at stack secrete chemokines which subsequently trigger fi broblasts residing adjacent migrate toward region. usefulness patterned, for performing mechanistic studies motility viability within laminar ventricle tissue heart. blood vessel tissue, ii) consumption these nutrients by metabolism. Most tissues body capillaries every ≈200 µm ensure suffi cient delivery all cells. [ 2 ] Cardi