Mapping quantitative trait loci (QTLs) for fatty acid composition in an interspecific cross of oil palm

作者: Rajinder Singh Rajinder Singh , SG Tan , JM Panandam , RA Rahman , LCL Ooi

DOI: 10.1186/1471-2229-9-114

关键词: Locus (genetics)Oleic acidPalmitoleic acidBotanyHorticultureQuantitative trait locusLinoleic acidElaeis oleiferaBiologyPalmitic acidMarker-assisted selection

摘要: Marker Assisted Selection (MAS) is well suited to a perennial crop like oil palm, in which the economic products are not produced until several years after planting. The use of DNA markers for selection such crops can greatly reduce number breeding cycles needed. With markers, informed decisions be made at nursery stage, regarding individuals should retained as stock, satisfactory agricultural production, and culled. trait associated with quality, measured terms its fatty acid composition, an important agronomic that eventually tracked using molecular markers. This will speed up production new improved palm planting materials. A map was constructed AFLP, RFLP SSR interspecific cross involving Colombian Elaeis oleifera (UP1026) Nigerian E. guinneensis (T128). framework generated male parent, T128, Joinmap ver. 4.0. In paternal (E. guineensis) map, 252 (199 38 15 SSR) could ordered 21 linkage groups (1815 cM). Interval mapping multiple-QTL model (MQM) (also known composite interval mapping, CIM) were used detect quantitative loci (QTLs) controlling quality (measured iodine value composition). At 5% genome-wide significance threshold level, QTLs (IV), myristic (C14:0), palmitic (C16:0), palmitoleic (C16:1), stearic (C18:0), oleic (C18:1) linoleic (C18:2) content detected. One genomic region on Group 1 appears influencing IV, C14:0, C16:0, C18:0 C18:1 content. Significant QTL C16:1, detected around same locus 15, thus revealing another major composition palm. Additional 3. minor C18:2 2. study describes first successful detection These constitute useful tools application programmes.

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