Identification of a major quantitative trait locus for resistance to maize rough dwarf virus in a Chinese maize inbred line X178 using a linkage map based on 514 gene-derived single nucleotide polymorphisms

摘要: Maize rough dwarf disease (MRDD) is a worldwide viral and causes significant yield losses in maize (Zea mays L.) production. In this study, we mapped characterized quantitative trait loci (QTL) conferring resistance to MRDD using 89 F8 recombinant inbred lines derived from cross between X178 (resistant parent) B73 (susceptible). The population was evaluated for Baoding, Hebei Province, China (a hot spot of incidence) under natural infection 2008 2009 artificial inoculation 2010. Genotypic variances severity index (DSI) were highly the population. Heritability estimates DSI evaluation 0.472 0.467 2009, respectively. linkage map constructed 514 gene-derived single nucleotide polymorphisms (SNPs) 72 simple sequence repeat markers, spanning genetic distance 1,059.72 cM with an average interval 1.8 adjacent markers. Multiple-QTL model mapping detected major QTL on chromosome 8, explaining 24.6–37.3% phenotypic variation across three environments. 2010, additional 10, 15.8% variation. 8 SNP markers (SNP31, SNP548, SNP284) co-located peak have potential further functional genomic analysis use molecular marker-assisted selection maize.