Natural Abundance Carbon‐13 Partially Relaxed Fourier Transform Nuclear Magnetic Resonance Spectra of Complex Molecules

摘要: Proton‐decoupled partially relaxed Fourier transform (PRFT) NMR of carbon‐13 in natural abundance was used to determine spin–lattice relaxation times (T1) individual carbons solutions cholesteryl chloride, sucrose, and adenosine 5′‐monophosphate (AMP) at 15.08 MHz 42°C. With the exception a few side‐chain groups, all protonated have T1 values less than 1 sec. Some on chloride show evidence internal reorientation up 2 Nonprotonated range 2–8 These are sufficiently short make ordinary very sensitive technique study complex molecules without need for spin‐echo refocusing schemes. Integrated intensities nuclear Overhauser enhancements prove that, except two three nonprotonated AMP, 13C nuclei these compounds relax mainly through 13C–1H dipolar interactions. Measured protonated...