Crosslinked duplex DNA nanogels that target specified proteins
作者： Yasuhiko Iwasaki , Jun-ichi Kondo , Akinori Kuzuya , Rui Moriyama
DOI: 10.1080/14686996.2016.1189798
关键词: Biophysics 、 Copolymer 、 Nanogel 、 Dispersity 、 Radical polymerization 、 Oligonucleotide 、 Dynamic light scattering 、 Polymer 、 Aptamer 、 Materials science 、 Nanotechnology
摘要: AbstractSpecific detection of protein biomarkers plays an important role in diagnostics and therapeutics. We have fabricated polymeric nanogels, which can specifically interact with the cancer biomarker thrombin to serve as a model. Two types 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymers bearing thrombin-binding oligonucleotide aptamer its complementary chain were independently synthesized by redox-initiated radical polymerization. These MPC polymers associate complimentary fashion due double strand formation oligonucleotides aqueous media, leading spontaneous spherical nanogels. Nanogel was confirmed dynamic light scattering (DLS) transmittance microscopy. The average size nanogel particles 124 ± 2 nm nanogels mono-dispersed (polydispersity index 0.21). Functional intercalators could be stably incorporated into through physical interaction between oligonucleotides. et...
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