Selecting specific PCR primers with MFEprimer
作者： Wubin Qu , Chenggang Zhang
DOI: 10.1007/978-1-4939-2365-6_15
关键词: Genetics 、 Specific primers 、 Polymerase chain reaction 、 DNA 、 Gene 、 Biology 、 Amplicon
摘要: Selecting specific primers is crucial for polymerase chain reaction (PCR). Nonspecific will bind to unintended genes and result in nonspecific amplicons. MFEprimer a program checking the specificity of PCR against background DNA. In this chapter, we introduce: (1) factors that affect primers; (2) principle its settings; (3) how use examine primers.
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