Design and Application of Multiplex PCR Seq for the Detection of Somatic Mutations Associated with Myeloid Malignancies.
作者： Naomi Park , George Vassiliou
DOI: 10.1007/978-1-4939-7142-8_6
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摘要: Targeted sequencing, in which only a selected set of genomic loci are sequenced, enables much higher coverage each target than what is obtained using whole genome or exome sequencing. Multiplex PCR offers simple and affordable technique for specific capture regions can be easily adapted to generate next-generation sequencing (NGS)-ready amplicons. Here we describe multiplex (MxPCR) approach capturing 13 leukemia-associated mutation hotspots followed by MiSeq that robust detection mutations with variant allele fraction (VAF) as low 0.8% (0.008) blood DNA.
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