A high-plex PCR approach for massively parallel sequencing.

作者: Tú Nguyen-Dumont , Bernard J. Pope , Fleur Hammet , Melissa C. Southey , Daniel J. Park

DOI: 10.2144/000114052

关键词:

摘要: … -customizable PCR-MPS technology that we call Hi-Plex (Figure 1). Hi-Plex panels can be … We have tested Hi-Plex in a 60-plex assay using all of the protein-coding and some of the 5…

参考文章(26)
Richard W Tothill, Maria A Doyle, Cliff Meldrum, Next-Generation Sequencing for Cancer Diagnostics: a Practical Perspective The Clinical biochemist. Reviews / Australian Association of Clinical Biochemists. ,vol. 32, pp. 177- 195 ,(2011)
Clare E. Holleley, Paul G. Geerts, Multiplex Manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR BioTechniques. ,vol. 46, pp. 511- 517 ,(2009) , 10.2144/000113156
Michael M. H. Yang, Ash Singhal, Shahrad Rod Rassekh, Stephen Yip, Patrice Eydoux, Christopher Dunham, Possible differentiation of cerebral glioblastoma into pleomorphic xanthoastrocytoma: an unusual case in an infant. Journal of Neurosurgery. ,vol. 9, pp. 517- 523 ,(2012) , 10.3171/2012.1.PEDS11326
Nazneen Rahman, Sheila Seal, Deborah Thompson, Patrick Kelly, Anthony Renwick, Anna Elliott, Sarah Reid, Katarina Spanova, Rita Barfoot, Tasnim Chagtai, Hiran Jayatilake, Lesley McGuffog, Sandra Hanks, D Gareth Evans, Diana Eccles, Breast Cancer Susceptibility Collaboration (UK), Douglas F Easton, Michael R Stratton, None, PALB2, which encodes a BRCA2-interacting protein, is a breast cancer susceptibility gene Nature Genetics. ,vol. 39, pp. 165- 167 ,(2007) , 10.1038/NG1959
Sean V. Tavtigian, Peter J. Oefner, Davit Babikyan, Anne Hartmann, Sue Healey, Florence Le Calvez-Kelm, Fabienne Lesueur, Graham B. Byrnes, Shu-Chun Chuang, Nathalie Forey, Corinna Feuchtinger, Lydie Gioia, Janet Hall, Mia Hashibe, Barbara Herte, Sandrine McKay-Chopin, Alun Thomas, Maxime P. Vallée, Catherine Voegele, Penelope M. Webb, David C. Whiteman, Suleeporn Sangrajrang, John L. Hopper, Melissa C. Southey, Irene L. Andrulis, Esther M. John, Georgia Chenevix-Trench, Rare, Evolutionarily Unlikely Missense Substitutions in ATM Confer Increased Risk of Breast Cancer American Journal of Human Genetics. ,vol. 85, pp. 427- 446 ,(2009) , 10.1016/J.AJHG.2009.08.018
Zhiyong Shen, Wubin Qu, Wen Wang, Yiming Lu, Yonghong Wu, Zhifeng Li, Xingyi Hang, Xiaolei Wang, Dongsheng Zhao, Chenggang Zhang, MPprimer: a program for reliable multiplex PCR primer design BMC Bioinformatics. ,vol. 11, pp. 143- 143 ,(2010) , 10.1186/1471-2105-11-143
Jay Shendure, Hanlee Ji, Next-generation DNA sequencing. Nature Biotechnology. ,vol. 26, pp. 1135- 1145 ,(2008) , 10.1038/NBT1486
Michal R. Schweiger, Martin Kerick, Bernd Timmermann, Marcus W. Albrecht, Tatjana Borodina, Dmitri Parkhomchuk, Kurt Zatloukal, Hans Lehrach, Genome-Wide Massively Parallel Sequencing of Formaldehyde Fixed-Paraffin Embedded (FFPE) Tumor Tissues for Copy-Number- and Mutation-Analysis PLoS ONE. ,vol. 4, pp. e5548- ,(2009) , 10.1371/JOURNAL.PONE.0005548
D.J. Park, F. Lesueur, T. Nguyen-Dumont, M. Pertesi, F. Odefrey, F. Hammet, S.L. Neuhausen, E.M. John, I.L. Andrulis, M.B. Terry, M. Daly, S. Buys, F. Le Calvez-Kelm, A. Lonie, B.J. Pope, H. Tsimiklis, C. Voegele, F.M. Hilbers, N. Hoogerbrugge, A. Barroso, A. Osorio, G.G. Giles, P. Devilee, J. Benitez, J.L. Hopper, S.V. Tavtigian, D.E. Goldgar, M.C. Southey, Rare mutations in XRCC2 increase the risk of breast cancer American Journal of Human Genetics. ,vol. 90, pp. 734- 739 ,(2012) , 10.1016/J.AJHG.2012.02.027
Xin-zhuan Su, Yimin Wu, C David Sifri, Thomas E Wellems, None, Reduced extension temperatures required for PCR amplification of extremely A+T-rich DNA. Nucleic Acids Research. ,vol. 24, pp. 1574- 1575 ,(1996) , 10.1093/NAR/24.8.1574