Methods and compositions for pcr using blocked and universal primers
作者： Karl Guegler , Eugene Spier
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摘要: Provided herein are methods and compositions for performing PCR with primers blocked 3′-ends that unblocked when these anneal to the template. The multiplexed can be used as real-time qPCR, end-point detection or enrichment method next generation sequencing (NGS). Also described improve sensitivity of mutation-specific targeting closely-spaced mutations.
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