Cross-platform compatibility of Hi-Plex, a streamlined approach for targeted massively parallel sequencing.

作者: Tú Nguyen-Dumont , Bernard J. Pope , Fleur Hammet , Maryam Mahmoodi , Helen Tsimiklis

DOI: 10.1016/J.AB.2013.07.046

关键词:

摘要: Although per-base sequencing costs have decreased during recent years, library preparation for targeted massively parallel remains constrained by high reagent cost, limited design flexibility, and protocol complexity. To address these limitations, we previously developed Hi-Plex, a polymerase chain reaction (PCR) strategy screening panels of genomic target regions. Here, demonstrate that Hi-Plex applied with hybrid adapters can generate suitable both the Ion Torrent TruSeq chemistries adjusting primer concentrations improves coverage uniformity. These results expand capabilities as an accurate, affordable, flexible, rapid approach various genetic applications.

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