The effect of pasteurization on transforming growth factor alpha and transforming growth factor beta 2 concentrations in human milk.

摘要: Transforming growth factor alpha (TGF-a) and beta 2 (TGF-s2) are present in human milk involved differentiation repair of neonatal intestinal epithelia. Heat treatment at 56°C has been shown effective for providing safe banked donor milk, with good retention other biologically active factors. The purpose our study was to determine the effect heat sterilization on TGF-a TGF-s2 concentrations milk. Twenty samples were collected from 20 lactating mothers polypropylene containers frozen -20°C transport or storage. Before by holder pasteurization, thawed divided into 1-mL aliquots. All heated an accurately regulated water bath until a holding temperature achieved, then held 30 minutes using constant agitation. Holding ranged 56.5°C 56.9°C. stored 4°C overnight analysis following day. concentration measured radioimmunoassay. Mean ± SD raw 119 50 pg/mL, range 57 234. mean treated 113 51 227. minimally affected overall loss 6.1%. Of 19 samples, 4 had increased 15 decreased after pasteurization (mean percent SEM: 94% 7% 72%-107%). acid-activated TGF- 32 enzyme-linked immunosorbent assay. 5624 5038 195 15480. heat-treated 5073 4646pg/mL, 181 15140. survived relatively little (0.6%): 18 11 7 99.4 6.7% 79%-120%). In conclusion, both TGF-132 well-preserved whole 56.5°C. relative increase some may be attributable release that cellular and/or fat compartments aqueous fraction These findings have implications regarding use as alternate source factors cytokines newborn gut when mother’s is unavailable.