The effect of glow discharge plasma surface modification of polymers on the osteogenic differentiation of committed human mesenchymal stem cells.
作者： Fackson Mwale , Hong Tian Wang , Valentin Nelea , Li Luo , John Antoniou
DOI: 10.1016/J.BIOMATERIALS.2005.11.006
关键词: Cell biology 、 Mesenchymal stem cell 、 Alkaline phosphatase 、 Immunology 、 Tissue engineering 、 Bone sialoprotein 、 Cellular differentiation 、 Osteocalcin 、 Surface modification 、 Polymer 、 Materials science
摘要: Little is known of the effect material surfaces on stem cell differentiation. The present study has addressed hypothesis that interaction mesenchymal cells (MSCs) with modified by glow discharge plasma a major regulator osteogenic We found biaxially oriented polypropylene (BOPP) treated in ammonia significantly reduced up-regulation expression marker genes, such as alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC). In contrast, ALP was up-regulated when cultured Nylon-6 polyamide (Ny-t) but substantially its pristine counterpart (Ny-p) day 3. On 7, down-regulated MSCs Ny-t although level up again 14. BSP expressed weakly 3, Ny-p. Its reached maximum 14 polystyrene control, while it cyclically Ny-t. Similarly, there slight increase OC were Ny-p compared to control. Thus, nature surface can directly influence differentiation, ultimately affecting quality new tissue formation BOPP-t suppressing
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