Immobilisation orientée du facteur de croissance de l'épiderme à des fins d'application en ingénierie tissulaire

摘要: RESUME Les implants generes par bio-ingenierie offrent des perspectives prometteuses en medecine regeneratrice grâce, entre autre, au developpement de supports mieux adaptes a la promotion la regeneration tissulaire. Plusieurs groupes recherche ont d’ailleurs developpe des techniques permettant d’immobiliser maniere non-orientee proteines afin de promouvoir l’adhesion et croissance cellulaire. Bien que reponses cellulaires aient pu etre observees, ces strategies couplage ne sont pas optimales en raison creation surfaces heterogenes l’alteration, dans nombreux cas, l’activite biologique ainsi greffees. Ces methodes fixation donc rapidement ete remplacees strategies favorisant le greffage oriente proteines. Pour ce faire, il est necessaire leur adjoindre une etiquette (Fc ou polyhistidine), puis faire interagir chimeres avec un support adequatement traite (proteines A/G ions metalliques). Recemment, une nouvelle approche basee sur l’interaction existant deux peptides, denommes E coil K coil, rapportee. peptides synthetiques possedent forte affinite et haute specificite l’un pour l’autre deja utilises diverses applications en genie biomedical biotechnologie (purification capture surface de biocapteurs). Ce systeme peptidique pourrait egalement etre utilise permettre l’immobilisation orientee telles facteurs croissance. Les devront alors etiquetees peptide tandis choisi devra presenter peptide coil complementaire prealablement greffe. Apres simple incubation, les devraient capables se fixer du materiau via coiled-coil ayant lieu peptides.----------ABSTRACT Bioengineered are becoming extremely promising with respect to the development of biocompatible scaffolds promote and sustain tissue regeneration. Several research groups have developed methods for the non-oriented immobilization growth factors (GF) ultimate goal modulating cell phenotype. Although unambiguous effects been reported randomly grafted ligands, these protocols suffer from several caveats such as heterogeneous surfaces on which protein bioactivity may be suboptimal. Consequently, new based interactions between affinity tags as Fc or polyhistidine solid supports, allowing oriented currently developed. Recently, heterodimeric coiled-coil system formed by two novo designed peptides (the coils) has characterized demonstrated be tool various biotechnology delivery due high affinity strong specificity that each others. In this work, we have decided take advantage specifically coil-tagged growth factor, EGF, in an fashion through its interaction coil partner will covalently immobilized. We selected EGF since factor is known to have major impact cellular proliferation during wound healing mechanisms, make it an excellent candidate engineering applications.