Proteases from the regenerating gut of the holothurian Eupentacta fraudatrix.

摘要: Four proteases with molecular masses of 132, 58, 53, and 47 kDa were detected in the digestive system holothurian Eupentacta fraudatrix. These displayed gelatinase activity characteristics zinc metalloproteinases. The 58 protease had similar inhibitor sensitivity to that mammalian matrix Zymographic assay revealed different lytic activities all four during intestine regeneration holothurian. 132 showed highest at first stage. During morphogenesis (stages 2–4 regeneration), was measured for 53 proteases. Inhibition exerts a marked effect on regeneration, which dependent time when 1,10-phenanthroline injections commenced. When metalloproteinases inhibited second stage restoration rates decreased. However, such an proved be reversible, inhibition ceased, previous rate recovered. is stage, completely abolished, animals die, suggesting early activation crucial triggering regenerative process holothurians. role processes holothurians discussed.