In defence of optical density ratios in 2-deoxyglucose autoradiography

摘要: The use of optical density ratios to describe changes in [14C]2-deoxyglucose uptake neuroanatomical mapping experiments has recently been criticized. It argued that a fixed ratio tissue isotope concentration does not yield constant but is dependent on the exposure time and absolute amounts used. Here it demonstrated such variations are due an artifact calculating ratio, which can easily be corrected provided film approaching saturation non-linearity exposure-density curve as previously suggested.