The pathway of glutamate oxidation by mitochondria isolated from different tissues.

摘要: Abstract 1. The pathway of glutamate oxidation by mitochondria isolated from rat liver, Ehrlich ascites cells, heart and pigeon-breast muscle has been investigated. 2. average inhibition malonate was 70% with rat-liver mitochondria, 82% ascites-cell 97% rat-heart sarcosomes more than pigeon-breast-muscle sarcosomes. Almost complete also found fluoride arsenite 3. Accumulation α-ketoglutarate during the rat-liver, negligible, ΔO/Δα-ketoglutarate being larger 100 in nearly all experiments. 4. Glutamic dehydrogenase activity, measured spectrophotometrically, very high moderate while no activity could be demonstrated or rate formation Krebs-Cohen dismutation ran roughly parallel to glutamic activity. 5. During absence inhibitors aspartate formed. Δ glutamate/Δ value, determined bacterial decar☐ylases, 1.0 for 1.1 mitochondria. 6. It is concluded that under experimental conditions used, predominant studied conversion means glutamate-oxaloacetate transaminase followed part Krebs-cycle lying between oxaloacetate. In transamination only one available rapid oxidation.