Multiplication of human chondrocytes with low seeding densities accelerates cell yield without losing redifferentiation capacity.

摘要: To treat a cartilage defect with tissue-engineering techniques, multiplication of donor cells is essential. However, during this in monolayer expansion culture chondrocytes will lose their phenotype and produce matrix inferior quality (dedifferentiation). Dedifferentiation occurs more extensively low seeding densities passaging. obtain good it important that the multiplicated regain cartilaginous (redifferentiation capacity). A "gold standard" for monolayer, respect to density passaging, lacking. In numerous available studies, various cell have been used, making comparison results these studies difficult. Therefore, we performed comparative study gain insight concerning effect passaging on capacity redifferentiate. From resulting data deduced which both sufficient fast, while retain As guideline calculated that, at minimum, 20-fold needed fill an average 4 cm(2) amount obtained. For used isolated ear from five children. Four different were ranging 3500 30000 cells/cm(2). The cultured four passages. expanded redifferentiate capacity) was studied after additional 3-week alginate beads assessed by glycosaminoglycan production immunohistochemical stainings collagen type I, II, elastin, fibroblast marker (11-fibrau). general, found decreasing yielded increase chondrocytes, but same time decreased dedifferentiation capacity. further analyzing our according proposed guidelines lower (20 times) reached less than higher densities. Importantly, redifferentiation preserved. It equal or even surpassed multiplied 20 times densities, required passages culture. Thus, purposes propose preferable.