Functional characteristics of reversibly immortalized hepatic progenitor cells derived from mouse embryonic liver.
作者： Yang Bi , Yun He , Jiayi Huang , Yuxi Su , Gao-Hui Zhu
DOI: 10.1159/000366340
关键词: Molecular biology 、 Stem cell 、 Cre recombinase 、 Embryonic stem cell 、 Progenitor 、 Hepatocyte 、 FOXA2 、 Biology 、 Liver Stem Cell 、 Progenitor cell
摘要: Background/Aims: Liver is a vital organ and retains its regeneration capability throughout adulthood, which requires contributions from different cell populations, including liver precursors intrahepatic stem cells. To overcome the mortality of hepatic progenitors (iHPs) in vitro, we aim to establish reversibly immortalized progenitor cells mouse embryonic liver. Methods Results: Using retroviral system stably express SV40 T antigen flanked with Cre/LoxP sites, repertoire iHP clones varied differentiation potential. The maintain long-term proliferative activity levels markers (Pou5f1/Oct4 Dlk) hepatocyte (AFP, Alb ApoB). Five representative hepatic/pancreatic transcription factors HNF3α/Foxa1, HNF3β/Foxa2, HNF4α/MODY1. Dexamethasone shown promote expression AFP TAT, along ICG-uptake glycogen storage functions clones. Cre-mediated removal reverses When are subcutaneously implanted athymic nude mice, no tumor formation observed for up 8 weeks. Conclusions: We demonstrate that established stable, reversible, non-tumorigenic progenitor-like cells, should be valuable studying organogenesis, metabolic regulations, lineage-specific differentiation.
doaj.org
core.ac.uk 参考文章(102)
Jinhua Wang, Hongmei Zhang, Wenwen Zhang, Enyi Huang, Ning Wang, Ningning Wu, Sheng Wen, Xian Chen, Zhan Liao, Fang Deng, Liangjun Yin, Junhui Zhang, Qian Zhang, Zhengjian Yan, Wei Liu, Zhonglin Zhang, Jixing Ye, Youlin Deng, Hue H Luu, Rex C Haydon, Tong-Chuan He, Feng Deng, None, Bone morphogenetic protein-9 effectively induces osteo/odontoblastic differentiation of the reversibly immortalized stem cells of dental apical papilla Stem Cells and Development. ,vol. 23, pp. 1405- 1416 ,(2014) , 10.1089/SCD.2013.0580
Yi Wang, Siqi Hong, Ming Li, Jiye Zhang, Yang Bi, Yun He, Xing Liu, Guoxin Nan, Yuxi Su, Gaohui Zhu, Ruidong Li, Wenwen Zhang, Jinhua Wang, Hongyu Zhang, Yuhan Kong, Wei Shui, Ningning Wu, Yunfeng He, Xian Chen, Hue H. Luu, Rex C. Haydon, Lewis L. Shi, Tong-Chuan He, Jiaqiang Qin, Noggin resistance contributes to the potent osteogenic capability of BMP9 in mesenchymal stem cells. Journal of Orthopaedic Research. ,vol. 31, pp. 1796- 1803 ,(2013) , 10.1002/JOR.22427
Hitoshi Niwa, Jun-ichi Miyazaki, Austin G. Smith, Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nature Genetics. ,vol. 24, pp. 372- 376 ,(2000) , 10.1038/74199
J Y Zhu, M Abate, P W Rice, C N Cole, The ability of simian virus 40 large T antigen to immortalize primary mouse embryo fibroblasts cosegregates with its ability to bind to p53. Journal of Virology. ,vol. 65, pp. 6872- 6880 ,(1991) , 10.1128/JVI.65.12.6872-6880.1991
Wolf D. Kuhlmann, Peter Peschke, Hepatic progenitor cells, stem cells, and AFP expression in models of liver injury. International Journal of Experimental Pathology. ,vol. 87, pp. 343- 359 ,(2006) , 10.1111/J.1365-2613.2006.00485.X
Gaurav A. Luther, Joseph Lamplot, Xiang Chen, Richard Rames, Eric R. Wagner, Xing Liu, Akash Parekh, Enyi Huang, Stephanie H. Kim, Jikun Shen, Rex C. Haydon, Tong-Chuan He, Hue H. Luu, IGFBP5 domains exert distinct inhibitory effects on the tumorigenicity and metastasis of human osteosarcoma Cancer Letters. ,vol. 336, pp. 222- 230 ,(2013) , 10.1016/J.CANLET.2013.05.002
Agnieszka Banas, Takumi Teratani, Yusuke Yamamoto, Makoto Tokuhara, Fumitaka Takeshita, Gary Quinn, Hitoshi Okochi, Takahiro Ochiya, Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytes Hepatology. ,vol. 46, pp. 219- 228 ,(2007) , 10.1002/HEP.21704
Atsunori Tsuchiya, Toshio Heike, Hisanori Fujino, Mitsutaka Shiota, Katsutsugu Umeda, Momoko Yoshimoto, Yasunobu Matsuda, Takafumi Ichida, Yutaka Aoyagi, Tatsutoshi Nakahata, Long-term Extensive Expansion of Mouse Hepatic Stem/Progenitor Cells in a Novel Serum-Free Culture System Gastroenterology. ,vol. 128, pp. 2089- 2104 ,(2005) , 10.1053/J.GASTRO.2005.03.030
Yuanyuan Du, Jinlin Wang, Jun Jia, Nan Song, Chengang Xiang, Jun Xu, Zhiyuan Hou, Xiaohua Su, Bei Liu, Tao Jiang, Dongxin Zhao, Yingli Sun, Jian Shu, Qingliang Guo, Ming Yin, Da Sun, Shichun Lu, Yan Shi, Hongkui Deng, Human Hepatocytes with Drug Metabolic Function Induced from Fibroblasts by Lineage Reprogramming Cell Stem Cell. ,vol. 14, pp. 394- 403 ,(2014) , 10.1016/J.STEM.2014.01.008
Kimberly D. Tremblay, Kenneth S. Zaret, Distinct populations of endoderm cells converge to generate the embryonic liver bud and ventral foregut tissues Developmental Biology. ,vol. 280, pp. 87- 99 ,(2005) , 10.1016/J.YDBIO.2005.01.003