Generation of human osteoclasts in stromal cell‐free and stromal cell‐rich cultures: differences in osteoclast CD11c/CD18 integrin expression

摘要: Osteoclasts form in the presence of macrophage colony-stimulating factor (M-CSF) and receptor activator Nfkappab ligand (RANKL), a membrane-bound differentiation that is now available as soluble recombinant molecule. Acquisition osteoclast phenotype [the alphavbeta3 subunit vitronectin (VNR)-, calcitonin (CTR)- F-actin ring-positive cells] associated with loss monocyte/macrophage-associated integrins, specifically CD11b, CD11c CD18. We hypothesized differences integrin adhesion molecule profile may exist osteoclasts generated stromal cell-rich stromal-free conditions. Unlike vivo, (resorbing) formed RANKL vitro, absence cells, co-expressed However, when were from peripheral blood mononuclear cells (PBMNCs) co-cultures murine bone marrow cell line 218 (which does not produce RANKL) RANKL, CD18 expressed by osteoclasts. These findings indicate persistent expression accounted for being presented required normal resorbing This study demonstrates potentially misleading information arise using data obtained they do completely reflect situation vivo.