SIN, A NOVEL DROSOPHILA PROTEIN THAT ASSOCIATES WITH THE RNA BINDING PROTEIN SEX-LETHAL

摘要: Sex-lethal (SXL) is an RNA binding protein that acts as a regulator of both alternative pre-mRNA splicing and translation. Because SXL must sometimes function at some distance from its sites, it believed interact with other proteins. We used yeast two-hybrid screen to isolate novel Drosophila protein, SIN (SXL interactor), interacts specifically SXL. A direct physical association was demonstrated in vitro, single domain sufficient for the interaction. shows high degree similarity mammalian unknown function. The cytogenetic location Sin 78A2-4. transcript, which abundant early embryos, appears be maternal origin.