Sex-lethal autoregulation requires multiple cis-acting elements upstream and downstream of the male exon and appears to depend largely on controlling the use of the male exon 5' splice site.

摘要: The on/off state of the binary switch gene Sex-lethal (Sxl), which controls somatic sexual development in Drosophila melanogaster, is regulated at level alternative splicing. In males, off, default splicing machinery produces nonfunctional mRNAs; females, on, autoregulatory activity Sxl proteins directs to produce functional mRNAs. We have used germ line transformation analyze mechanism and Our results demonstrate that a blockage employed autoregulation. However, contrast transformer, appears function by preventing interaction factors with 3' splice site, different strategy (i) Multiple cis-acting elements, both upstream downstream male exon, are required. (ii) These elements distant from sites they regulate, suggesting protein cannot autoregulation directly competing for sites. (iii) 5' site exon be dominant regulation while plays subordinate role.