Sequences involved in the control of Adenovirus L1 alternative RNA splicing

摘要: Abstract During an adenovirus infection the expression of mRNA from late region L1 is temporally regulated at level alternative 3' splice site selection to produce two major mRNAs encoding 52,55K and IIIa polypeptides. The proximal (52,55K) used all times infectious cycle whereas distal (IIIa) exclusively after infection. We show that a single A branch nucleotide located position -23 in splicing A's positions -21 -22 are splicing. Both sites were active vitro nuclear extracts prepared uninfected HeLa cells. However, efficiency was only approximately 10% This difference activity correlated with reduced affinity IIIa, relative 52,55K, for polypyrimidine tract binding proteins. Reversing order on tandem pre-mRNA resulted almost exclusive indicating presentation important outcome Based our results we suggest cis competition model where compete common RNA factor(s). may represent mechanism by which regulated.