作者: William S. Marshall , Richard S. Nishioka
关键词: In vitro 、 Biophysics 、 Cell density 、 Biology 、 Marine fish 、 Gillichthys 、 Biochemistry 、 Chloride 、 Mitochondrion 、 Stratified epithelium
摘要: Mitochondria-rich cells in the skin of marine teleost, Gillichthys mirabilis, were found to be ultrastructurally similar typical chloride-secreting fish gill, but had a tall, spindlelike shape due thickness stratified epithelium. The fluorophore, dimethylaminostyrylethyl-pyridiniumiodide (DASPEI), was used visualize and count chloride so that cell density could regressed against vitro short-circuit current (ISC) measured on same tissue. regression (r2 = 0.76; n 72) demonstrated are responsible for anion transport across Grillichthys skin.