Method for genetic detection using interspersed genetic elements: a multiplexed DNA analysis system
作者： Sudhir Sinha
DOI:
关键词: Multiplex 、 Genetics 、 Identification (biology) 、 Biology 、 Amelogenin 、 Computational biology 、 Retrotransposon 、 Amplicon 、 Allele 、 Gene duplication 、 DNA
摘要: By utilizing a Mini-Primer strategy targeting the target site duplication (TSD) sequence of retrotransposons, INNUL markers, which include SINEs, LINEs, and SVAs, can be effectively used as markers for human identification bio-ancestry studies regardless size inserted element. The amplicons INNULs difference between allelic states reduced substantially such that these have utility analyzing high low quality DNA samples. A 15 RE marker Amelogenin (for sex determination) multiplex single tube amplification DNA, in four color detection was successfully designed. provided power discrimination suitable forensic paternity analysis. In addition, sensitivity enable identity on anthropological Depending distribution alleles global populations, selected testing or studies.
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