A change in the cerebrosides and sulfatides in a demyelinating nervous system. Development of the methodology and study of multiple sclerosis and Wallerian degeneration.

摘要: This report described a new method for the microanalysis of sphingolipids and its application characterization cerebrosides sulfatides in multiple sclerosis brain rat sciatic nerves undergoing Wallerian degeneration. Tissue was extracted with isopropanol/hexane (20:78), total lipids obtained were subjected to benzoylation-desulfation. A portion this directly analyzed by silica-column high performance liquid chromatography determination nonhydroxycerebroside, hydroxycerebroside, nonhydroxysulfatide, hydroxysulfatide. Another fractionated thin-layer chromatography, spots corresponding sphingolipid derivatives eluted. The material from each spot reverse phase homolog composition. With procedure concentrations compositions measured plaque, periplaque, normal-appearing white matter brains patients degenerated distal nerve transection. One piece plaque studied contained only 1.86, 2.76, 0.60, 0.45 nmol nonhydroxysulfatide hydroxysulfatide/mg protein, respectively. These are less than 1% those found normal matter. Periplaques contain these between levels degenerative 10-20% below third day after severed about 70% 10 days. rate decrease lessened ten days 55 both degenerating similar control. implications findings advantages analytical discussed.