A novel synthetic-genetic-array-based yeast one-hybrid system for high discovery rate and short processing time.
作者： Chung-Shu Yeh , Zhifeng Wang , Fang Miao , Hongyan Ma , Chung-Ting Kao
关键词: DNA 、 Computational biology 、 Chromatin immunoprecipitation 、 Transcription factor 、 Hybrid system 、 Transcription (biology) 、 Biology 、 Yeast 、 Gene regulatory network 、 Synthetic genetic array
摘要: Eukaryotic gene expression is often tightly regulated by interactions between transcription factors (TFs) and their DNA cis targets. Yeast one-hybrid (Y1H) one of the most extensively used methods to discover these interactions. We developed a high-throughput meiosis-directed yeast system using Magic Markers synthetic genetic array analysis. The has factor-DNA interaction discovery rate twice as high conventional diploid-mating approach processing time nearly one-tenth haploid-transformation method. also offers highest accuracy in identifying TF-DNA that can be authenticated vivo chromatin immunoprecipitation. With unique features, this Y1H particularly suited for constructing novel comprehensive genome-scale regulatory networks various organisms.
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