Search for retroviral related DNA polymorphisms using RAPD PCR in schizophrenia.
作者： Paromita Deb , Timothy A. Klempan , Richard L. O’Reilly , Shiva M. Singh
DOI: 10.1016/S0925-4439(98)00103-3
关键词: DNA 、 Context (language use) 、 Retrovirus 、 Genetics 、 Human genome 、 Biology 、 RAPD 、 Polymorphism (computer science) 、 Primer (molecular biology) 、 Genetic marker
摘要: Abstract Random amplification of polymorphic DNA (RAPD) is widely used to detect polymorphisms in many organisms. Individual (or strain) specific amplified bands are generated with single or pairs primers PCR reactions and can serve as genetic markers. We have this method generate a large number reproducible primers, random retroviral related, on 92 human samples. Theoretically, RAPD presents logical approach for assessing variability among individuals. ten related (12, 20 22 bp) eight (10 assess individual differences the context testing hypothesis schizophrenia. Three discordant monozygotic twins, four full sibs 53 schizophrenic individuals 25 their unrelated matched controls were analyzed. Ten these resulted total approx. 850 (65–110 per primer). Almost all identical each However, results inconclusive respect The general lack polymorphism study may argue mechanisms other than rearrangements such inversions, associated evolution genome.
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