Knockdown of p53 suppresses Nanog expression in embryonic stem cells
作者： Essam Mohamed Abdelalim , Ikuo Tooyama
DOI: 10.1016/J.BBRC.2013.12.030
关键词: Gene knockdown 、 Embryonic stem cell 、 Stem cell 、 RNA 、 Tumor suppressor gene 、 Downregulation and upregulation 、 Molecular biology 、 DNA damage 、 Homeobox protein NANOG 、 Biology
摘要: Abstract Mouse embryonic stem cells (ESCs) express high levels of cytoplasmic p53. Exposure mouse ESCs to DNA damage leads activation p53, inducing Nanog suppression. In contrast earlier studies, we recently reported that chemical inhibition p53 suppresses ESC proliferation. Here, confirm signaling is involved in the maintenance self-renewal. RNA interference-mediated knockdown induced downregulation p21 and defects Furthermore, resulted a significant expression at 24 48 h post-transfection. also caused reduction Oct4 Conversely, exposure higher control siRNA-treated than cells. These data show absence damage, required for self-renewal by regulating expression.
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