The voltage-sensitive sodium channel from rabbit skeletal muscle. Chemical characterization of subunits.
作者： R.H. Roberts , R.L. Barchi
DOI: 10.1016/S0021-9258(18)61653-9
关键词: Amino acid 、 Skeletal muscle 、 ATP synthase alpha/beta subunits 、 G alpha subunit 、 Sodium 、 Biochemistry 、 Molecular biology 、 Beta (finance) 、 Chemistry 、 Protein subunit 、 Gel electrophoresis
摘要: The alpha and beta subunits of the rabbit skeletal muscle sodium channel have been separated isolated preparatively under denaturing conditions. In this channel, subunit is not linked covalently to subunit. subjected amino acid carbohydrate analysis. Both are heavily glycosylated (alpha = 26.5%, 29.7% by weight) with N-acetylneuraminic N-acetylhexosamines representing predominant monosaccharides in each. Enzymatic deglycosylation neuraminidase endoglycosidase F yielded single core peptides approximately 209 kDa for 26.5 Based on known composition, molecular masses are, therefore, 285 37.5 beta, respectively. Using subunits, we calibrated our protein-labeling system dodecyl sulfate-polyacrylamide gel electrophoresis determined stoichiometry channel; native preparation, molar ratio alpha:beta 1 : 1.
sci-hub.st 参考文章(22)
James T. Cassidy, George W. Jourdian, Saul Roseman, The sialic acids. VI. Purification and properties of sialidase from Clostridium perfringens. Journal of Biological Chemistry. ,vol. 240, pp. 3501- 3506 ,(1965) , 10.1016/S0021-9258(18)97171-1
Vernon N. Reinhold, [19] Gas-liquid chromatographic analysis of constituent carbohydrates in glycoproteins Methods in Enzymology. ,vol. 25, pp. 244- 249 ,(1972) , 10.1016/S0076-6879(72)25021-2
D J Messner, W A Catterall, The sodium channel from rat brain. Role of the beta 1 and beta 2 subunits in saxitoxin binding. Journal of Biological Chemistry. ,vol. 261, pp. 211- 215 ,(1986) , 10.1016/S0021-9258(17)42455-0
S D Kraner, J C Tanaka, R L Barchi, Purification and functional reconstitution of the voltage-sensitive sodium channel from rabbit T-tubular membranes. Journal of Biological Chemistry. ,vol. 260, pp. 6341- 6347 ,(1985) , 10.1016/S0021-9258(18)88977-3
T Gonoi, SJ Sherman, WA Catterall, Voltage clamp analysis of tetrodotoxin-sensitive and -insensitive sodium channels in rat muscle cells developing in vitro. The Journal of Neuroscience. ,vol. 5, pp. 2559- 2564 ,(1985) , 10.1523/JNEUROSCI.05-09-02559.1985
R L Barchi, R D Gordon, J M Casadei, Immunoaffinity isolation of Na+ channels from rat skeletal muscle. Analysis of subunits. Journal of Biological Chemistry. ,vol. 261, pp. 4318- 4323 ,(1986) , 10.1016/S0021-9258(17)35663-6
R P Hartshorne, W A Catterall, The sodium channel from rat brain. Purification and subunit composition. Journal of Biological Chemistry. ,vol. 259, pp. 1667- 1675 ,(1984) , 10.1016/S0021-9258(17)43460-0
D J Messner, W A Catterall, The sodium channel from rat brain. Separation and characterization of subunits. Journal of Biological Chemistry. ,vol. 260, pp. 10597- 10604 ,(1985) , 10.1016/S0021-9258(19)85127-X
Alain LOMBET, Michel LAZDUNSKI, Characterization, solubilization, affinity labeling and purification of the cardiac Na+ channel using Tityus toxin γ FEBS Journal. ,vol. 141, pp. 651- 660 ,(1984) , 10.1111/J.1432-1033.1984.TB08241.X
Lawrence W. Elmer, Bonnie J. O'Brien, Thomas J. Nutter, Kimon J. Angelides, Physicochemical characterization of the alpha-peptide of the sodium channel from rat brain. Biochemistry. ,vol. 24, pp. 8128- 8137 ,(1985) , 10.1021/BI00348A044