Isolation and in vitro expansion of human colonic stem cells
作者： Peter Jung , Toshiro Sato , Anna Merlos-Suárez , Francisco M Barriga , Mar Iglesias
DOI: 10.1038/NM.2470
关键词: Biology 、 Cell biology 、 Amniotic stem cells 、 Population 、 Endothelial stem cell 、 Cancer stem cell 、 Pathology 、 Stem cell 、 Cell type 、 Adult stem cell 、 Cell
摘要: Here we describe the isolation of stem cells human colonic epithelium. Differential cell surface abundance ephrin type-B receptor 2 (EPHB2) allows purification different types from colon mucosa biopsies. The highest EPHB2 levels correspond to epithelial with longest telomeres and elevated expression intestinal (ISC) marker genes. Moreover, using culturing conditions that recreate ISC niche, a substantial proportion EPHB2-high can be expanded in vitro as an undifferentiated multipotent population.
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