Increased sensitivity of a direct fluorescent antibody test for Legionella pneumophila in bronchoalveolar lavage samples by immunomagnetic separation based on BioMags
作者： Shneh Sethi , Mitchell T. Gore , Krishan K. Sethi
DOI: 10.1016/J.MIMET.2007.05.006
关键词: Legionella pneumophila 、 Bacteria 、 Immunoglobulin G 、 Bronchoalveolar lavage 、 Microbiology 、 Staining 、 Biology 、 Positive patient 、 Chromatography 、 Direct fluorescent antibody 、 Immunomagnetic separation 、 Microbiology (medical) 、 Molecular biology
摘要: Abstract In the present study, immunomagnetic separation of Legionella pneumophila from mock bronchoalveolar lavage (BAL) fluid samples, which were artificially spiked with L. pneumophila, and culture positive patient BAL was achieved using BioMags (superparamagnetic particles) loaded purified rabbit immunoglobulin G specific for pneumophila. Bacteria binding onto BioMag-immunomatrix directly stained a species-specific DFA reagent examined under fluorescence microscope. BioMag-based (BIMS) followed by staining (BIMS–DFA) could correctly identify all 20 (100%) samples low numbers (2 × 102 CFU) Cultures be recovered 15 (75%) these 5 (25%) failed to yield cultures. Both BIMS–DFA methods showed 100% results when containing high bacterial load (104 CFU) tested. The findings true specimens retrospectively indicated that is significantly more sensitive detecting than conventional cytospin method (cytospin–DFA). Out 25 tested, 7 (28%) proved negative cytospin–DFA whereas detected specimens. It suggested procedure increases sensitivity testing in large volume such as fluids.
elsevier.com
sci-hub.se 参考文章(38)
A. Gilmour, Max Sussman, R. G. Kroll, New techniques in food and beverage microbiology. Blackwell Scientific Publications Ltd.. ,(1993)
Gordon A McFeters, None, Drinking water microbiology : progress and recent developments ,(2011)
W L Thacker, B B Plikaytis, H W Wilkinson, Identification of 22 Legionella species and 33 serogroups with the slide agglutination test. Journal of Clinical Microbiology. ,vol. 21, pp. 779- 782 ,(1985) , 10.1128/JCM.21.5.779-782.1985
P H Edelstein, K B Beer, J C Sturge, A J Watson, L C Goldstein, Clinical utility of a monoclonal direct fluorescent reagent specific for Legionella pneumophila: comparative study with other reagents. Journal of Clinical Microbiology. ,vol. 22, pp. 419- 421 ,(1985) , 10.1128/JCM.22.3.419-421.1985
L H Gosting, K Cabrian, J C Sturge, L C Goldstein, Identification of a species-specific antigen in Legionella pneumophila by a monoclonal antibody. Journal of Clinical Microbiology. ,vol. 20, pp. 1031- 1035 ,(1984) , 10.1128/JCM.20.6.1031-1035.1984
O Olsvik, T Popovic, E Skjerve, K S Cudjoe, E Hornes, J Ugelstad, M Uhlén, Magnetic separation techniques in diagnostic microbiology. Clinical Microbiology Reviews. ,vol. 7, pp. 43- 54 ,(1994) , 10.1128/CMR.7.1.43
H H Kessler, F F Reinthaler, A Pschaid, K Pierer, B Kleinhappl, E Eber, E Marth, Rapid detection of Legionella species in bronchoalveolar lavage fluids with the EnviroAmp Legionella PCR amplification and detection kit. Journal of Clinical Microbiology. ,vol. 31, pp. 3325- 3328 ,(1993) , 10.1128/JCM.31.12.3325-3328.1993
Sydney M. Finegold, Paul H. Edelstein, Richard D. Meyer, Laboratory Diagnosis of Legionnaires' Disease1–4 The American review of respiratory disease. ,vol. 121, pp. 317- ,(2015) , 10.1164/ARRD.1980.121.2.317
David W. Fraser, Theodore R. Tsai, Walter Orenstein, William E. Parkin, H. James Beecham, Robert G. Sharrar, John Harris, George F. Mallison, Stanley M. Martin, Joseph E. McDade, Charles C. Shepard, Philip S. Brachman, , Legionnaires' Disease: Description of an Epidemic of Pneumonia The New England Journal of Medicine. ,vol. 297, pp. 1189- 1197 ,(1977) , 10.1056/NEJM197712012972201
Angela D.M. Kashuba, Charles H. Ballow, Legionella urinary antigen testing: potential impact on diagnosis and antibiotic therapy. Diagnostic Microbiology and Infectious Disease. ,vol. 24, pp. 129- 139 ,(1996) , 10.1016/0732-8893(96)00010-7