The α1-Na/K Pump does not Mediate the Involvement of Ouabain in the Development of Hypertension in Rats

摘要: The Na/K pump of vascular smooth muscle cells (VSMC) and renal epithelial (REC) is viewed as a target digitalis endogenous ouabain (EO), leading to the development hypertension. In this study, we compared effect on activity intracellular content monovalent cations in VSMC REC obtained from rats, humans dogs. rat aorta, inhibited maximal measured rate 86 Rb influx Na + -loaded cells, with an ID 50 ~20-30 µM without any differences between two strains normotensive rats (WKY BN.lx) three substrains spontaneously hypertensive (SHR). Half-maximal inhibition inner medullary collecting duct was observed at ~20 ouabain. contrast half-maximal human coronary arteries Madin-Darby canine kidney seen ~0.03 0.1 ouabain, respectively. At concentrations...