Alignment of inducible vascular progenitor cells on a micro-bundle scaffold improves cardiac repair following myocardial infarction

摘要: Ischemic heart disease is still the leading cause of death even with advancement pharmaceutical therapies and surgical procedures. Early vascularization in ischemic critical for a better outcome. Although stem cell therapy has great potential cardiovascular regeneration, ideal type delivery method cells have not been resolved. We tested new approach by induced vascular progenitor (iVPCs) grown on polymer micro-bundle scaffolds rat model myocardial infarction. iVPCs partially reprogrammed from endothelial (ECs) had potent angiogenic were able to simultaneously differentiate into smooth muscle (SMCs) ECs 2D culture. Under hypoxic conditions, also secreted cytokines such as growth factor (VEGF) basic fibroblast (bFGF) measured enzyme-linked immunosorbent assay (ELISA). A longitudinal micro-scaffold made poly(lactic-co-glycolic acid) was sufficient iVPCs. Co-cultured SMCs aligned well scaffold similarly vessels. 3D cell/polymer micro-bundles formed micro-scaffolds transplanted myocardium infarction (MI) ligation left anterior descending artery. Our vivo data showed that higher survival, retention engraftment than free promoted cardiomyocyte survival free iVPCs. Moreover, iVPC/polymer treatment improved cardiac function (ejection fraction fractional shortening, endocardial systolic volume) echocardiography, increased vessel density, decreased size [endocardial epicardial infarct (scar) length] untreated controls at 8 weeks after MI. conclude are promising cell-based designed regeneration disease.