Establishment and characterization of a new Epstein-Barr virus transformed cell line from a human B cell lymphoma
作者： Ottmar Janssen , Barbara Heinze , Claus R. Bartram , Michael Pawlita , Thomas Binder
DOI: 10.1007/BF01720278
关键词: Virology 、 B cell 、 Cell culture 、 B-cell lymphoma 、 Epstein–Barr virus 、 Naive B cell 、 Clone (B-cell biology) 、 Monoclonal 、 Biology 、 Monoclonal antibody
摘要: We have established a new cell line from patient with centrocytic B lymphoma. Highly purified peripheral blood cells DUL (WBC counts 158,000/µl) were infected in vitro Epstein-Barr virus (EBV), and CD 20+ cloned into 96 well culture plates the aid of sorter autoclone device. As shown by GTG-banding Southern blot analysis, outgrowing EBV-positive clones had same chromosomal abnormalities identical monoclonal IgH gene rearrangement as original EBV-genome-negative leukemic clone. Surface marker analysis panel antibodies revealed patterns on EBV-negative -positive clones, exception PCA 1 (reactive plasma cells) which was negative freshly explanted but positive EBV-converted clones.
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