Permissiveness of bovine epithelial cells from lung, intestine, placenta and udder for infection with Coxiella burnetii.

摘要: Ruminants are the main source of human infections with obligate intracellular bacterium Coxiella (C.) burnetii. Infected animals shed high numbers C. burnetii by milk, feces, and birth products. In goats, shedding latter route coincides replication in epithelial (trophoblast) cells placenta, which led us to hypothesize that generally implicated We therefore aimed at analyzing interactions bovine host (1) entry site (lung epithelium) govern immune responses (2) gut, udder placenta decisive for quantity pathogen excretion. Epithelial cell lines [PS (udder), FKD-R 971 (small intestine), BCEC (maternal placenta), F3 (fetal BEL-26 (lung)] were inoculated strains Nine Mile I (NMI) NMII different cultivation conditions. The exhibited permissiveness While maintaining viability, allowed highest rates formation large cell-filling containing vacuoles. Intestinal showed an enhanced susceptibility invasion but supported only intermediate levels. Lung placental also internalized bacteria strikingly smaller numbers. any cells, both failed trigger a substantial IL-1β, IL-6 TNF-α response. mammary particular, may serve as niche vivo without alerting host’s