Modeling red cabbage seed extract effect on Penicillium corylophilum: Relationship between germination time, individual and population lag time.

作者: Stéphane Dagnas , Maria Gougouli , Bernard Onno , Konstantinos P. Koutsoumanis , Jeanne-Marie Membré

DOI: 10.1016/J.IJFOODMICRO.2015.07.008

关键词: Red cabbagePopulationPenicillium corylophilumSpore germinationLagBrassicaGerminationBiologyHorticultureSporeBotany

摘要: The inhibitory effect of a red cabbage seed extract on germination time, individual (single spore) and population lag time Penicillium corylophilum was studied. First, to compare the biological variability single spore times under stressful conditions, data were collected at levels varying from 0 10 mg/g (150 spores observed in each trial germination, ca 50 experiment). Experiments performed malt agar 25 °C, pH 5.2, aw 0.99. data, without any transformation, statistically analyzed; several probability distribution functions used fit cumulated spores. In both cases, best obtained with Normal distribution. parallel, level (ca 2000 per trial) for same range plant extract. Not surprisingly, difference between could be explained by stochastic process. More interestingly, it shown that did not correspond required 95% spores, but much longer time. Finally, deduced statistical analysis, completed microscopic observations, affected mainly hyphal elongation (and then time) germination. Next, secondary models developed quantify median times, times. Minimum Inhibitory Concentrations (MICs) estimated. It MIC P. depend inoculum load. Application allowed us conclude conditions our experiment, addition enabled extension two weeks.

参考文章(45)
Philippe Dantigny, Iavor Tchobanov, Maurice Bensoussan, Marcel H Zwietering, None, Modeling the effect of ethanol vapor on the germination time of Penicillium chrysogenum. Journal of Food Protection. ,vol. 68, pp. 1203- 1207 ,(2005) , 10.4315/0362-028X-68.6.1203
John I. Pitt, Ailsa D. Hocking, Penicillium and Related Genera Food Spoilage Microorganisms. pp. 169- 273 ,(2009) , 10.1007/978-0-387-92207-2_7
Daiana Garcia, Antonio J. Ramos, Vicente Sanchis, Sonia Marín, Modelling mould growth under suboptimal environmental conditions and inoculum size Food Microbiology. ,vol. 27, pp. 909- 917 ,(2010) , 10.1016/J.FM.2010.05.015
Stéphane Dagnas, Bernard Onno, Jeanne-Marie Membré, Modeling growth of three bakery product spoilage molds as a function of water activity, temperature and pH. International Journal of Food Microbiology. ,vol. 186, pp. 95- 104 ,(2014) , 10.1016/J.IJFOODMICRO.2014.06.022
L COROLLER, V GUERROT, V HUCHET, Y LEMARC, P MAFART, D SOHIER, D THUAULT, Modelling the influence of single acid and mixture on bacterial growth. International Journal of Food Microbiology. ,vol. 100, pp. 167- 178 ,(2005) , 10.1016/J.IJFOODMICRO.2004.10.014
M.E. Guynot, A.J. Ramos, V. Sanchis, S. Marín, Study of benzoate, propionate, and sorbate salts as mould spoilage inhibitors on intermediate moisture bakery products of low pH (4.5-5.5). International Journal of Food Microbiology. ,vol. 101, pp. 161- 168 ,(2005) , 10.1016/J.IJFOODMICRO.2004.11.003
Maria Gougouli, Konstantinos P. Koutsoumanis, Relation between germination and mycelium growth of individual fungal spores. International Journal of Food Microbiology. ,vol. 161, pp. 231- 239 ,(2013) , 10.1016/J.IJFOODMICRO.2012.12.006
Maria Gougouli, Konstantinos P. Koutsoumanis, Modeling germination of fungal spores at constant and fluctuating temperature conditions. International Journal of Food Microbiology. ,vol. 152, pp. 153- 161 ,(2012) , 10.1016/J.IJFOODMICRO.2011.07.030
Philippe Dantigny, Sonia Marín, Marco Beyer, Naresh Magan, Mould germination: data treatment and modelling. International Journal of Food Microbiology. ,vol. 114, pp. 17- 24 ,(2007) , 10.1016/J.IJFOODMICRO.2006.11.002