作者: Stéphane Dagnas , Maria Gougouli , Bernard Onno , Konstantinos P. Koutsoumanis , Jeanne-Marie Membré
DOI: 10.1016/J.IJFOODMICRO.2015.07.008
关键词: Red cabbage 、 Population 、 Penicillium corylophilum 、 Spore germination 、 Lag 、 Brassica 、 Germination 、 Biology 、 Horticulture 、 Spore 、 Botany
摘要: The inhibitory effect of a red cabbage seed extract on germination time, individual (single spore) and population lag time Penicillium corylophilum was studied. First, to compare the biological variability single spore times under stressful conditions, data were collected at levels varying from 0 10 mg/g (150 spores observed in each trial germination, ca 50 experiment). Experiments performed malt agar 25 °C, pH 5.2, aw 0.99. data, without any transformation, statistically analyzed; several probability distribution functions used fit cumulated spores. In both cases, best obtained with Normal distribution. parallel, level (ca 2000 per trial) for same range plant extract. Not surprisingly, difference between could be explained by stochastic process. More interestingly, it shown that did not correspond required 95% spores, but much longer time. Finally, deduced statistical analysis, completed microscopic observations, affected mainly hyphal elongation (and then time) germination. Next, secondary models developed quantify median times, times. Minimum Inhibitory Concentrations (MICs) estimated. It MIC P. depend inoculum load. Application allowed us conclude conditions our experiment, addition enabled extension two weeks.