Red blood cell three-dimensional morphometry by quantitative phase microscopy

摘要: In humans, healthy mature erythrocytes or Red Blood Cells (RBCs) have globule structure and mostly important they lack a cell nucleus most organelles, thus RBC is an envelope filled of uniform transparent liquid. Abnormal RBCs may be fragmented shaped like teardrops, crescents, needles, variety other forms deviating from their regular ordinary shape. Here we show that seeing erythrocyte-ensemble as nanolens-array, detection abnormal cells can made rapidly efficiently without recurring to subjective shape analysis image by the doctor sophisticated processing tools, but rather exploiting alterations affecting lens-focusing properties. Demonstration how aberrations affect focusing properties given Hartmann- Shack approach Zernike polynomial-fitting, occurs for wavefront aberration correction in adaptive modern astronomic telescopes. The results concept biological lens could addressed revolutionary integration between photonics biology fast blood pre-screening performed proposed approach.