System and method for antigen structure-independent detection of antigens captured on antibody arrays

作者: George Dacai Liu

DOI:

关键词: Antibody microarrayVirologyAntibodyAntigenMolecular biologyChemistry

摘要: The present invention provides a system and method for detecting antigens captured on an antibody array. comprises the following steps of providing array having at least two antibodies, contacting with sample containing one antigen that may be by antibodies disposed array, agent specifically binds to antigen-bound thereby can detected independent structures antigens. In preferred embodiment, C1q is used as detect antibodies.

参考文章(21)
Michael Tainsky, Sorin Draghici, Madhumita Chatterjee, Neoepitope detection of disease using protein arrays ,(2006)
G. Marqués, L.C. Antón, E. Barrio, A. Sánchez, S. Ruiz, F. Gavilanes, F. Vivanco, Arginine residues of the globular regions of human C1q involved in the interaction with immunoglobulin G. Journal of Biological Chemistry. ,vol. 268, pp. 10393- 10402 ,(1993) , 10.1016/S0021-9258(18)82214-1
Karel Newman, Jane Schmidt, Paul Wegfahrt, Immunoassays for determining vitamin b12, and reagents and kits therefor ,(1993)
Allan D. Pronovost, Jan W. Pawlak, Theodore T. Sand, Cathy A. Bacquet, Assay device for one step detection of analyte ,(1994)
Elazar Rabbani, Jannis Stavrianopoulos, James Gerard Wetmur, Samuel B. Abrams, Analyte detection by means of energy transfer ,(1987)
Sun Ai Raillard, Claus Krebber, Mark Welch, Kurt Vogel, S. Christopher Davis, Jeremy Minshull, Biosensors, reagents and diagnostic applications of directed evolution ,(2001)