Metabolic profile of pitaya (Hylocereus polyrhizus (F.A.C. Weber) Britton & Rose) by UPLC-QTOF-MSE and assessment of its toxicity and anxiolytic-like effect in adult zebrafish.

摘要: Abstract Pitaya is a Cactacea with potential for economic exploitation, due to its high commercial value and functional components – such betalains, oligosaccharides phenolic compounds. Although the biological activities of pitaya have been studied using in vivo vitro models (anti-inflammatory antiproliferative activities, as example), anxiolytic-like effect still unexplored. Therefore, aim this work was perform characterization pulp peel (Hylocereus polyrhizus (F.A.C. Weber) Britton & Rose) UPLC-QTOF-MSE, assess toxicity adult zebrafish (Danio rerio). The results showed 16 15 compounds (in peel, respectively), including maltotriose, quercetin-3-O-hexoside, putatively identified by UPLC-QTOF-MSE. Thus, no both tested (Vero cell lines model, LC50 ˃ 1 mg/mL); significant anxiolytic activity, since treated fish reduced permanence clear zone (Light Dark Test) compared that control, exhibiting anxiolytic-simile diazepam. However, these effects were pre-treatment flumazenil suggesting are anxiolytics agents mediated via GABAergic system. These findings suggested H. polyrizhus has developing an alternative plant-derived therapy. In addition, (which waste food industry) should be regarded valuable product, which value-added ingredient anxiety disorders.