Attenuation of quorum sensing-mediated virulence of Acinetobacter baumannii by Glycyrrhiza glabra flavonoids.
作者： Nidhi Bhargava , Sukhvinder P Singh , Anupam Sharma , Prince Sharma , Neena Capalash
DOI: 10.2217/FMB.15.107
关键词: Biofilm 、 Acinetobacter baumannii 、 Microbiology 、 Biology 、 Glycyrrhiza 、 Enzyme 、 Quorum sensing 、 Virulence 、 Quorum Quenching 、 Autoinducer
摘要: Aim: To develop an alternative quorum quenching therapy against multidrug-resistant Acinetobacter baumannii. Methods & results: Activity-guided partially purified fraction (F1) from Glycyrrhiza glabra significantly (p < 0.05) reduced sensing regulated virulence factors of A. baumannii viz. motility, biofilm formation and production antioxidant enzymes. Mechanistically, F1 downregulated the expression autoinducer synthase gene, abaI, consequently (92%) 3-OH-C12-HSL as determined by ESI–MS. Q-TOF Q-TRAP analyses suggested presence flavonoids licoricone, glycyrin glyzarin active ingredients. Conclusion: This is first report on activity G. linked to its that abaI attenuated
futuremedicine.com
sci-hub.se 参考文章(53)
Halkare Suryanarayana Vasavi, Ananthapadmanabha Bhagwath Arun, Punchapady-Devasya Rekha, Anti-quorum sensing activity of Psidium guajava L. flavonoids against Chromobacterium violaceum and Pseudomonas aeruginosa PAO1. Microbiology and Immunology. ,vol. 58, pp. 286- 293 ,(2014) , 10.1111/1348-0421.12150
Michael Lynch, Howard Kuramitsu, Expression and role of superoxide dismutases (SOD) in pathogenic bacteria. Microbes and Infection. ,vol. 2, pp. 1245- 1255 ,(2000) , 10.1016/S1286-4579(00)01278-8
Jeffrey H Miller, Experiments in molecular genetics ,(1972)
C Fuqua, S C Winans, Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes. Journal of Bacteriology. ,vol. 178, pp. 435- 440 ,(1996) , 10.1128/JB.178.2.435-440.1996
P N Viswanathan, Poonam Kakkar, Ballabh Das, A modified spectrophotometric assay of superoxide dismutase. Indian Journal of Biochemistry & Biophysics. ,vol. 21, pp. 130- 132 ,(1984)
Shashikant M Prajapati, Bhupesh R Patel, None, Phyto-Pharmacological Perspective of Yashtimadhu (Glycyrrhiza Glabra LINN.) - A Review International Journal of Pharmaceutical & Biological Archive. ,vol. 4, ,(2013)
John Makemson, Anatol Eberhard, Kalai Mathee, Simple electrospray mass spectrometry detection of acylhomoserine lactones Luminescence. ,vol. 21, pp. 1- 6 ,(2006) , 10.1002/BIO.873
Danyelle R. Long, Julia Mead, Jay M. Hendricks, Michele E. Hardy, Jovanka M. Voyich, 18β-Glycyrrhetinic Acid Inhibits Methicillin-Resistant Staphylococcus aureus Survival and Attenuates Virulence Gene Expression Antimicrobial Agents and Chemotherapy. ,vol. 57, pp. 241- 247 ,(2013) , 10.1128/AAC.01023-12
Paul D. Stapleton, Peter W. Taylor, Methicillin resistance in Staphylococcus aureus: mechanisms and modulation. Science Progress. ,vol. 85, pp. 57- 72 ,(2002) , 10.3184/003685002783238870
Thimmaraju Rudrappa, Harsh P. Bais, Curcumin, a known phenolic from Curcuma longa, attenuates the virulence of Pseudomonas aeruginosa PAO1 in whole plant and animal pathogenicity models. Journal of Agricultural and Food Chemistry. ,vol. 56, pp. 1955- 1962 ,(2008) , 10.1021/JF072591J