Fentanyl inhibits proliferation and invasion via enhancing miR‑302b expression in esophageal squamous cell carcinoma

摘要: Fentanyl is one of the most commonly used intravenous anesthetic agents during cancer resection surgery, but effect fentanyl on esophageal squamous cell carcinoma (ESCC) remains unclear. The aim present study was to investigate involvement microRNA 302b (miR-302b) in anti-proliferation and anti-invasion effects ESCC. In study, proliferation, apoptosis invasion were detected using MTT assays, flow cytometry Transwell assays ESCC Eca109 TE1 lines. Subsequently, expression miR-302b determined reverse transcription-quantitative polymerase chain reaction (RT-qPCR). RT-qPCR western blot analysis performed order evaluate ErbB4, a target miR-302b. Furthermore, anti-miR inhibit fentanyl-treated cells role malignant behaviors. inhibited proliferation dose- time-dependent manner. Following exposure for 48 h, exhibited increased decreased invasion. upregulated expression, downregulated ErbB4 expression. Finally, loss technique reversed two Taken together, results indicated that inhibits through upregulation